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KMID : 0613820020120060821
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Journal of Life Science
2002 Volume.12 No. 6 p.821 ~ p.834
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Phylogenetic position of five Korean strains of Alexandrium tamarense (Dinophyceae), based on internal transcribed spacers ITS1 and ITS2 including nuclear-encoded 5.8S rRNA gene sequences
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Cho Eun-Seob
Lee Sam-Geun
Kim Ik-Soo
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Abstract
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In order to measure the inter- and intraspecific genetic divergences within the genus Alexandrium, the variations within the internal transcribed spacer (ITS1 and ITS2) regions and 5.8S ribosomal RNA gene of eight Alexandrium species were examined for 33 strains from diverse geographical locations by direct sequencing. Five isolates of A. tamarense (AT-2, AT-6, AT-10, AT-A and AT-B) from Jinhae Bay, Korea were found to be completely identical to a Japanese strain OFX151-A. The length of the amplified ITS1-5.8S-ITS2 region varied from 481 nucleotides (in A. margalefi) to 528 nucleotides (in A. affine CU1-1). ITS1 and ITS2 nucleotide lengths were negatively correlated, whereas a positive correlation was found between their G+C content. The degree of sequence divergence ranged from 0.3% (1 bp) to a maximum of 53% (305 bp). Pairwise sequence comparisons revealed a small degree of divergence between A. tamarense and A. fundyense isolates (1.2 - 2.3% = 6-12 bp), but a high degree of divergence between A. tamarense and A. catenella (19.8% = 102 bp), and between A. catenella and A. fundyense (19.7%). Although most nodes were weakly supported by bootstrap values, some types tend to form independent molecular groups. A. catenella isolates also formed an independent molecular sub-group, with relatively strong bootstrap values (94% or 85% and 79% or 98%, respectively in PAUP and NJ trees). Interestingly, A. cohorticula and A. frateculus always clustered within the same sub-group, this result being supported by strong bootstrap values. Our results indicate that the ITS regions provide useful informations on hierarchical population genetic structure and a high phylogenetic resolution in intraspecific and interspecific Alexandrium population.
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KEYWORD
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Alexandrium tamarense, ITS, molecular analysis, phylogeny, PSP, taxonomy
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